Leveraging Cas13a's trans-cleavage on RNA G-quadruplexes for amplification-free RNA detection
Description
In this study, we investigated Cas13a's efficacy in trans-cleaving RNA G-quadruplexes (rG4s) as an alternative to ssRNA reporters in CRISPR-Cas13a diagnostics. Our findings demonstrate enhanced efficiency due to the structural arrangement of rG4s